Establishment and Characterization of a Transplantable Dibutylnitrosamine-induced Mouse Bladder Tumor Line FCB1

Bladders were removed from IF x C57BL F[ hybrid mice that had been treated for 36 weeks with dibutylnitrosamine and implanted s.c. into similar hybrid mice to await the appearance of tumors. One of the tumors induced by this method has been serially transplanted for 42 generations. This has been designated FCB and is a rapidly growing transitional cell carcinoma with a stable morphology, as shown by light and electron microscopy. The tumor grows in a variety of sites and can be transplanted to either C57BL or IF mice. It will grow rapidly in vitro as a monolayer and re-forms solid tumor tissue upon reimplantation. The growth characteristics and morphology of the FCB tumor are described and discussed.